Testability

Are the Methods of Biological Evolution Testable?

Proponents of evolution often complain of the unequal treatment that it receives in comparison to other scientific theories. They point out that theories of gravity, the atom, the cell etc. are rarely if ever challenged while the opposite is true of evolution. Why? An obvious starting point to answer that question is to point out a critical difference. The others are operational theories of phenomena occurring at the present time and, therefore, readily studied and observed; on the other hand evolution is an historical theory that claims to know how certain events occurred in the distant past, events that were, obviously, not observed and cannot be replicated by science. (For example, scientists cannot make living things.) This critical difference means that operational theories are verifiable to a high degree of certainty by scientific testing while the main claims of evolution are arguably not testable and, therefore, not verifiable.

That realization has caused more than one scientist to ask the question, “What do we know to be true about evolution?” Or put another way, “What are the facts of evolution?” In science these questions bring to mind terms such as “verifiable”, “testable” and “ potentially falsifiable”. Claims made by scientists about the origin, operation and structure of natural systems must be tested in multiple ways by many scientists to determine their truth or falsity. Testability, verification, falsification, reproducibility of results are the central characteristics of the scientific method. Hypotheses that remain untested after a reasonable period of time after their formulation should be discarded. However, the tendency in evolution has been to keep untestable hypotheses on the front burner until a better one comes along. If that does not happen, the hypothesis’s longevity often spanning many generations of scientists gives it a gravitas that allows it to become part of the evolutionary culture. If this happens often enough, a theory is constructed as a collection of related but untested hypotheses which is defended as being a scientific theory when in reality it is a myth. This type of theory fends off all opponents by requiring that they demonstrate the falsity of hypotheses which in the case of evolution is impossible because it would require knowledge of events in the distant past. It is the turning of the scientific method on its head by basing the acceptance of hypotheses not on verification via testing but instead on the lack of falsifying evidence. It is analogous to a legal trial where the prosecution’s evidence is presumed true and the defendant is presumed guilty unless the defense can prove otherwise.

Back to the original question, “What do we know to be true about evolution?” Evolutionary scientists claim that a few processes produced all of life’s organisms from one ancestral cell. Has that claim been substantially verified by testing? The processes are the following:

1) Mutations accumulate to produce major changes in organisms

2) Endosymbiotic formation of the eukaryotic cell

3) According to The National Academy of Science publication Science and Creationism, complex molecular systems can be formed in the following way : “Natural selection can bring together parts of a system for one function at one time and then, at a later time, recombine those parts with other systems of components to produce a system that has a different function.”

4) Complex anatomical systems can develop by evolving through of a series of stages, each stage being a completely functional system, but each being more advanced than the system from which it evolved.

1) Mutations Accumulate to Produce Major Changes in Organisms

Darwin’s gradualistic version of evolution formulated in the mid-19th century persists to this day. He believed that a mechanism existed for making small changes in organisms that would accumulate to produce major change. He had observed species of finches and believed that the process that caused the differences in the finches could, given enough time, produce insects, fish, birds, humans, in short all of life’s organisms. In his day the molecular and cellular makeup of organisms was unknown and, therefore, Darwin was unable to propose what the mechanism for change might be. That had to wait until the middle of the 20th century when mutations in the DNA of reproductive cells were suggested as the means for producing small changes that could be passed on to progeny.

This is the most important hypothesis of evolution---mutations chosen by a natural selection process have accumulated over hundreds of millions of years to produce all organisms from an ancestral cell. This is accepted as fact and stated without reservation in virtually all presentations of evolution But has this hypothesis been tested? Can it be tested? Could it have been tested before DNA was discovered?

The Fossil Test
Although Darwin had no knowledge of DNA, he believed his gradualistic version of evolution would become obvious once the earth’s sedimentary strata revealed their fossil content. He believed that all forms of life were connected by “infinitely numerous transitional links” with “every stratum full of such intermediate links”. Obviously if Darwin were alive today, he would be extremely disappointed by the fossil record. Hundreds of thousands of fossils have been collected and not one example exists of features, such as legs, wings and backbones developing gradually. Whenever they appear in the fossil record, they are fully formed. Actually and not unexpectedly, fossils mimic living organisms in that they do not form a continuum. Living things are not connected by Darwin’s “infinitely numerous transitional links” and neither are fossils.

The paucity of transitional links has caused many evolutionist to accept a modified, gradualistic version, one that would not produce many transitional links. The modified version is called “punctuated equilibrium”. It hypothesizes that for most of their lifetime organisms of the past did not change but when they did, they did it rapidly and the change occurred in small populations. Therefore, there never were many transitional forms and that explains their absence from the fossil record. It could happen like this. Suppose turtles evolved from a particular group of frogs living in a small pond that effectively isolated them from other frogs. The mutations that gave the frogs turtle-like features occurred rapidly and to at least one frog of each sex who through mating passed the mutations on to their progeny. Eventually the Adam and Eve of turtles emerged from the pond and set forth to populate the earth, and none of the transitional frog-turtles (frotles?) was preserved as a fossil.

Punctuated equilibrium was first proposed in the 1970’s and has become accepted by many evolutionists. Can it be verified by testing? Obviously not. The evidence for it is no evidence, namely the absence of transitional links.

The DNA Test
How about experimenting with DNA to see if sequences of changes induced in it will produce major beneficial changes in organisms? If fossils contained DNA, perhaps the hypothesis could be tested by comparing their DNA. But they do not. Experiments on living organisms that might test the hypothesis are a fool’s errand because of the enormous complexity of cellular systems. A single gene can be changed in thousands of ways and the ramifications of most changes are unknown. The likelihood that a researcher can induce a sequence of mutations in succeeding generations of an organism that will provide beneficial changes, as evolution claims happened innumerable times in the past, is so small that it stifles attempts to try it. As a result the mutation hypothesis has not and cannot be tested by this method. The degree of untestability is complete applying not only to major changes in organisms but also to those at the species level. In other words mutations as the cause of trivial differences such as that of a gray squirrel and brown one are no more testable than those of a fish and turtle. That is evident in the following challenge of Lynn Margulis who is known for the endosymbiotic model of the eukaryotic cell. In one of her many public speeches she challenges molecular biologists to give an unambiguous example of the formation of a single new species by the accumulation of mutations. Her challenge goes unmet.1 The mutation hypothesis remains untested and, if anything, the fossil record shows it much more likely to be false than true. However, its 150 yearlong existence has elevated it to scientific fact and its is stated without reservation in countless textbooks and other expositions of evolution

2) The Endosymbiotic Formation of the Eukaryotic Cell

The eukaryotic cell differs from prokaryotes (bacteria) in that many of its functions are compartmentalized in small units called organelles. For example, the DNA is stored in an organelle called the nucleus and the molecular fuel is produced in organelles called mitochondria. Since all multi celled plants and animals consist of eukaryotic cells, the eukaryotic cell is considered more advanced then the prokaryotic cell and, therefore, is believed to have evolved from it. But how?

In the 1970’s Lynn Margulis proposed that eukaryotic cells resulted from a symbiotic relationship between two prokaryotic cells. A small prokaryote entered a larger prokaryote and the smaller one evolved into a mitochondrion and the larger one evolved all the features of a eukaryotic cell. Of course, this took a long time, possibly a billion years and many, many cell divisions. After being met with initial derision, the hypothesis was widely accepted as similarities between prokaryotes and mitochondria became known. For example, both divide; both contain DNA in ring form; they have similar sizes. But in spite of these similarities, prokaryotic cells and mitochondria are vastly different. In addition, eukaryotic cells are vastly different from prokaryotic cells.

Here are a few of the differences between eukaryotic and prokaryotic cells. Besides the nucleus and mitochondria, eukaryotic cells have several other organelles. The endoplasmic reticulum and the Golgi complex are two large organelles that are active in the synthesis of many different kinds of proteins. Lysosomes are waste disposal plants, containing enzymes for dismantling a variety of cellular fragments. Peroxisomes are more specialized waste disposal plants. Chloroplasts in plant cells change light into a chemical fuel.

Because a eukaryotic cell in comparison to a prokaryote is like New York City to Small Town, USA, a complex transportation system exists in the former. This includes a variety of small vessels that encapsulate and transport cargo within the cell. In addition molecular motors pull organelles and molecular fragments along a complex network of filaments strung across the cell. All this intracellular movement requires an elaborate mail and address system so that cargo reaches the correct destination.
Eukaryotic cells also contain a cytoskeleton, a complex network of three kinds of filaments that gives the cell some rigidity, compartmentalizes the cell’s interior and serves as roads in the transportation system. The filaments are dynamic in that they can disassemble and reassemble in different places for different purposes as they do when separating chromosomes during cell division.


Finally since all activities in cells are tightly coordinated, eukaryotic cells have an enormously complex system that directs and controls that activity.

Is Margulis’s hypothesis for the formation of the eukaryotic cell testable?
Definitely not! Because no scientist is capable of demonstrating the existence of a sequence of molecular events that can or did change the prokaryote-cell-within-a-prokaryote-cell system to a eukaryotic cell. And the profound molecular differences strongly suggest that it never happened. As for experimental testing, is anyone foolish enough to inject a large prokaryote with a smaller one and expect it to produce a mitochondrion? Yet this untestable hypothesis appears in biology textbooks as the truth with no disclaimers.

After the mitochondrion hypothesis was made, a similar idea was proposed for the formation of chloroplasts in eukaryotic plant cells. It suggested that plant cells were formed by two invasions of smaller prokaryotes into a large one. The first invasion produced a mitochondrion and the later invasion by a different kind of prokaryote produced a chloroplast with the capability of changing light into a molecular fuel. This untestable hypothesis is very effectively displayed in biology textbooks with a series of pictures showing the small cells crawling inside a larger cell and changing to mitochondria and chloroplasts.

3) According to The National Academy of Science publication Science and Creationism, complex molecular systems can be formed in the following way : “Natural selection can bring together parts of a system for one function at one time and then, at a later time, recombine those parts with other systems of components to produce a system that has a different function.”
Of the four hypothesized processes as to how evolution works, this is probably the most recent. It is a response to the challenge to evolution by the concept of irreducibly complex systems as formulated by the biochemist Michael Behe.2 He demonstrates that many cellular systems are irreducibly complex, which means they consist of several complex interdependent parts all of which are necessary for the system to function. Such systems could not evolve in a stepwise manner. Behe cites the mousetrap as an example of a system where all the parts are necessary for the trap to work. The removal of any part renders the trap useless. So to explain the existence of such systems evolutionists are hypothesizing the fantastically fortuitous scenario of several complex structures evolving independently for various purposes and then, at a later time, coming together and changing in just the right way so that they can function as a unit. This seems unbelievable but apparently it is believable enough to some who dare to put it in print. Obviously this claim is not testable and is not worthy of any scientist let alone The National Academy of Science.

4) Complex anatomical systems can develop by evolving through of a series of stages, each stage being a completely functional system, but each being more advanced than the system from which it evolved.
According to The National Academy of Science publication Science and Creationism eyes evolved in this manner. It shows a spot of light sensitive cells in a flatworm evolving into an insect eye with a light focusing lens which, in turn, evolves into the camera eye of vertebrates. This is the same explanation that Darwin used 150 years ago when challenged to explain how eyes evolved. He can be excused for his simplistic explanation because a molecular description of vision was unknown in his day. However, now that it is known, this kind of anatomical explanation which does not address the underlying molecular systems is inexcusable. Could it be that this omission is deliberate? Because such discussions would reveal the enormous complexity of these systems and cause people to question an evolutionary origin? In any event there is no explanation for the origin of sight that can be verified by testing.

The analysis of these pivotal hypotheses of evolution, the ones that claim to explain how it happened, demonstrates that they do not meet the criteria of being scientific because they are not testable. Hence they cannot be verified or falsified. They are beliefs masquerading as science. They should not be taught in science courses. But since we have gotten to the regrettable situation that the teaching of evolution in the public schools is mandated, the least we can do is provide an accurate account by telling the bad along with the good. Textbook presentations are over the top favorable, leaving no room for doubt that evolution has occurred. They may be worthy of a shady used car salesman who extols the positive while consciously hiding the fatal flaws under the hood but they are not worthy of scientists.

Competing theories regarding how life came to exist on earth, such as creationism and intelligent design, have supernatural aspects. Because of that they should not be taught in science classes. Evolution has won the day in the courts because it is the only purely natural theory. However, being purely natural does not suffice to make evolution a scientific theory. Only verification by testing of its central claims can do that. Therefore, evolutionary scientists should be required to list the truths of evolution. If they cannot or will not do that, then they are admitting that evolution is a myth and should not be taught in science courses in the public schools.

1. Behe, M. J. Darwin’s Black Box, (New York: Simon and Schuster, 1996), p. 26.

2. Ibid., p. 39.

 

 

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